WB
Western blot analysis of ADORA2B using anti-ADORA2B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HEK293 whole cell lysates, Lane 2: U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADORA2B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
IF analysis of ADORA2B using anti-ADORA2B antibody. ADORA2B was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ADORA2B Antibody at a dilution of 1:100. Omnimabs® 488 Goat Anti-Mouse IgG(H&L) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U251 cells using anti-ADORA2B antibody. Overlay histogram showing U251 cells stained with anti-ADORA2B antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADORA2B Antibody at 1:100 dilution for 30 min at 20°C. Omnimabs® 488 Goat Anti-Mouse IgG(H&L) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ADORA2B |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ADORA2B recombinant protein (Position: N145-L328) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADORA2B |
|---|---|
| Gene Synonyms | ADORA2 |
| Gene Full Name | adenosine A2b receptor |
| Gene Summary | This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| Alternative Names | adenosine A2b receptor Adenosine receptor A2b ADORA2 ADORA2B |
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of ADORA2B using anti-ADORA2B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HEK293 whole cell lysates, Lane 2: U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADORA2B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
IF analysis of ADORA2B using anti-ADORA2B antibody. ADORA2B was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ADORA2B Antibody at a dilution of 1:100. Omnimabs® 488 Goat Anti-Mouse IgG(H&L) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U251 cells using anti-ADORA2B antibody. Overlay histogram showing U251 cells stained with anti-ADORA2B antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADORA2B Antibody at 1:100 dilution for 30 min at 20°C. Omnimabs® 488 Goat Anti-Mouse IgG(H&L) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
