The adipocyte enhancer binding protein 1 (AEBP1) is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. AEBP1 may play a role in differentiated vascular smooth muscle cells.1) Layne, M.D., et al., (1998) J.Biol.Chem.273(25), 15654-15660.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AEBP1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
AEBP1 antibody can be used for detection of AEBP1 by ELISA at 1:1562500. AEBP1 antibody can be used for detection of AEBP1 by western blot at 0.2-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Intestine.
Application
Antibody used in IHC on Human Spermatophore.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
AEBP1 antibody can be used for detection of AEBP1 by ELISA at 1:1562500. AEBP1 antibody can be used for detection of AEBP1 by western blot at 0.2-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AEBP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.