Application
ApplicationApplication
'Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PRMT3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemisty. BC = breast carcinoma'Application
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with the primary PRMT3 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.Application
'Western blot analysis of anti-PRMT2 Pab in whole HL60 cell lysate. PRMT3 (arrow) was detected using purified Pab (lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.'| Product Name | PRMT3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PRMT3 - ChIP GradeArginine methylation is an irreversible post translational modification that has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions. |
| Immunogen | Synthetic peptide: STKTHWKQTVFLLEKPF, corresponding to amino acids 456-472 of Human PRMT 3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWBChIP |
| ChIP: Use 10μl for 10^6 cells. ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Predicted molecular weight: 57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Protein Arginine N-Methyltransferase 3 |
|---|---|
| Target | PRMT3 |
| Cellular Localization | Cytoplasmic |
Application
Application
Application
'Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PRMT3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemisty. BC = breast carcinoma'
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with the primary PRMT3 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
Application
'Western blot analysis of anti-PRMT2 Pab in whole HL60 cell lysate. PRMT3 (arrow) was detected using purified Pab (lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.'| Application Notes | ChIP: Use 10μl for 10^6 cells. ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Predicted molecular weight: 57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.1% sodium azide |
