Application
Western blot analysis of PRMT4 in cell lysates from HeLa cells at 0.5 ug /ml. Twenty microgram of HeLa cell lysate was loaded per well of mini gel.| Product Name | PRMT4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PRMT4Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT4 belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain 2 of the SRC (steroid receptor coactivator) GRIP1, whereas p300 interacts with activation domain 1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation. |
| Immunogen | Synthetic peptides, corresponding to amino acids 45-69 (NGEIQRHAEQQALRLEVRAGPDAAG) and 595-608 (SPMSIPTNTMHYGS) of Human PRMT4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 60 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human,Mouse |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | PRMT4 |
|---|---|
| Target | PRMT4 |
| Cellular Localization | Nuclear |
Application
Western blot analysis of PRMT4 in cell lysates from HeLa cells at 0.5 ug /ml. Twenty microgram of HeLa cell lysate was loaded per well of mini gel.| Application Notes | WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 60 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS, 0.1mg/ml BSA |
