Application
Western blot analysis of AFAP1 in Hela cell lysate with AFAP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | AFAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AFAP1 Antibody: AFAP, AFAP110, AFAP-110, AFAP, Actin filament-associated protein 1, 110 kDa actin filament-associated protein |
| Product description | AFAP1 Antibody: The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src. AFAP1 has a fundamental role in actin cytoskeleton arrangement. It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3-binding (SH3-binding) motif, and several SH2-binding motifs. It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis.1) Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892-900. |
| Immunogen | AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1. The immunogen is located within amino acids 30 - 80 of AFAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AFAP1 |
|---|---|
| Alternative Names | AFAP1 Antibody: AFAP AFAP110 AFAP-110 AFAP Actin filament-associated protein 1 110 kDa actin filament-associated protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (78%) |
| Tissue Specificity | Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members. |
Application
Western blot analysis of AFAP1 in Hela cell lysate with AFAP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AFAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AFAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
