Application
PRMT5 antibody [N1C1] immunoprecipitates PRMT5 protein in IP experiments. IP samples: Raji whole cell extract A. 35 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 μg PRMT5 antibody [N1C1] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000Application
Cross-linked ChIP was performed with K562 chromatin extract and 5 μg of either control rabbit IgG or anti-PRMT5 antibody. The precipitated DNA was detected by PCR with primer set targeting to γ-globin promoter.| Product Name | PRMT5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRMT5 (protein arginine methyltransferase 5)Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRMT5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,IP,WB,ChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, Chimpanzee, BovineMouse (98%), Rat (97%), Pig (98%), Rhesus Monkey (93%), Chimpanzee (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HRMT1L5 antibody, IBP72 antibody, JBP1 antibody, SKB1 antibody, SKB1Hs antibody, PRMT5 antibody, SKB1 homolog antibody, protein arginine N-methyltransferase 5 antibody, shk1 kinase-binding protein 1 homolog antibody, 72 kDa ICln-binding protein antibody, H |
|---|---|
| Molecular Weight(MW) | 73 kDa (note) (3) |
Application
PRMT5 antibody [N1C1] immunoprecipitates PRMT5 protein in IP experiments. IP samples: Raji whole cell extract A. 35 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 μg PRMT5 antibody [N1C1] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000
Application
Cross-linked ChIP was performed with K562 chromatin extract and 5 μg of either control rabbit IgG or anti-PRMT5 antibody. The precipitated DNA was detected by PCR with primer set targeting to γ-globin promoter.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
