Application
Immunocytochemistry of AFAP1L in A549 cells with AFAP1L antibody at 5 μg/mL.Application
Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.Application
Immunofluorescence of AFAP1L in A549 cells with AFAP1L antibody at 20 μg/mL.| Product Name | AFAP1L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | AFAP1L Antibody: The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4). Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.1) Snyder BN, Cho Y, Qian Y, et al. AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. Eur. J. Cell Biol. 2011; 90:376-89 |
| Immunogen | AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1. The immunogen is located within amino acids 70 - 120 of AFAP1L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,ICC,IF,WB |
| AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AFAP1L1 |
|---|---|
| Alternative Names | AFAP1L Antibody: Actin filament-associated protein 1-like 1 AFAP1-like protein 1 |
| Sequence Similarities | 2) Cat. No. 17-003 - A549 Cell Slide |
| Tissue Specificity | At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members. |
Application
Immunocytochemistry of AFAP1L in A549 cells with AFAP1L antibody at 5 μg/mL.
Application
Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.
Application
Immunofluorescence of AFAP1L in A549 cells with AFAP1L antibody at 20 μg/mL.| Application Notes | AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | AFAP1L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide. |
