Application
Immunohistochemical analysis of paraffin-embedded Mahlavu xenograft, using PRMT5 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: Raji 7.5% SDS PAGE SKB1 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PRMT5 antibody at 1:200 dilution.Application
PRMT5 antibody [N3C3] detects PRMT5 protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PRMT5 antibody [N3C3] dilution: 1:500.Application
PRMT5 antibody [N3C3] detects PRMT5 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PRMT5 antibody [N3C3] dilution: 1:500.| Product Name | PRMT5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRMT5 (protein arginine methyltransferase 5)Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRMT5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Japanese medaka, Xenopus laevis, Pig, Rhesus Monkey, Chimpanzee, BovineRat (98%), Zebrafish (85%), Japanese medaka (86%), Xenopus laevis (91%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HRMT1L5 antibody IBP72 antibody JBP1 antibody SKB1 antibody SKB1Hs antibody PRMT5 antibody SKB1 homolog antibody protein arginine N-methyltransferase 5 antibody shk1 kinase-binding protein 1 homolog antibody 72 kDa ICln-binding protein antibody H |
|---|---|
| Molecular Weight(MW) | 73 kDa (note) (7) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded Mahlavu xenograft, using PRMT5 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: Raji 7.5% SDS PAGE SKB1 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PRMT5 antibody at 1:200 dilution.
Application
PRMT5 antibody [N3C3] detects PRMT5 protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PRMT5 antibody [N3C3] dilution: 1:500.
Application
PRMT5 antibody [N3C3] detects PRMT5 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PRMT5 antibody [N3C3] dilution: 1:500.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
