Application
PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 μg of PRMT7 antibody 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 µg of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PRMT7 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PRMT7 antibody at 1:500 dilution.Application
Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 μg of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.Application
PRMT7 antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | PRMT7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRMT7 (protein arginine methyltransferase 7)Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ10640 antibody, KIAA1933 antibody, PRMT7 antibody, histone-arginine N-methyltransferase PRMT7 antibody, protein arginine N-methyltransferase 7 antibody, [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody, myelin basic protein-arginine N- |
|---|---|
| Molecular Weight(MW) | 78 kDa (note) (4) |
| Cellular Localization | Cytoplasm , cytosol , Nucleus |
Application
PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 μg of PRMT7 antibody 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 µg of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PRMT7 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PRMT7 antibody at 1:500 dilution.
Application
Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 μg of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.
Application
PRMT7 antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
