Application
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
AFTPH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | AFTPH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | AFTPH may play a role in membrane trafficking.1) Lui-Roberts, W.W., et al. Mol. Biol. Cell 19(12):5072-5081(2008) |
| Immunogen | This AFTPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 844-871 amino acids from the C-terminal region of human AFTPH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AFTPH |
|---|---|
| Alternative Names | Aftiphilin AFTPH AFTH |
| Molecular Weight(MW) | 102 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
AFTPH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
