Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (Phospho-Ser197).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197).| Product Name | AFX (phospho Ser197) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.1) Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. Essers MA, et al. EMBO J 2004 Nov. 11. Brownawell AM, (2001) Mol Cell Biol; 21 (10): 3534-46. Kops GJ, et al. (1999) Nature; 398 (6728): 630-4. |
| Immunogen | AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX. |
| Modification | p-Ser197 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IF,IHC,WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO4 |
|---|---|
| Alternative Names | AFX AFX1 MLLT7 EIF2A AFX FOXO4 Afxh |
| Molecular Weight(MW) | 65 kDa |
| Tissue Specificity | This antibody detects endogenous level of AFX only when phosphorylated at serine 197. |
| Entrez Gene | 4303 |
|---|---|
| Protein Accession | NP_001164402.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (Phospho-Ser197).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
