Application
Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 μg/mL.Application
Western blot analysis of FOXO4 in HeLa cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | FOXO4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXO4 Antibody: AFX, AFX1, MLLT7, AFX, Forkhead box protein O4, Fork head domain transcription factor AFX1 |
| Product description | FOXO4 Antibody: FOXO4 is a ubiquitously expressed protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In the absence of PI3K/AKT activation, FOXO4 localizes in the nucleus where it functions as a transcription factor. FOXO4 can also be phosphorylated by JNK following induction of reactive oxygen species (ROS), resulting in transcriptional activation and the induction of a negative feedback mechanism to counteract the ROS. It is through this mechanism that FOXO4 is thought to sensitize cancer cells to doxorubicin-mediated toxicity.1) Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99. |
| Immunogen | FOXO4 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FOXO4. The immunogen is located within amino acids 30 - 80 of FOXO4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| FOXO4 antibody can be used for detection of FOXO4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO4 |
|---|---|
| Alternative Names | FOXO4 Antibody: AFX AFX1 MLLT7 AFX Forkhead box protein O4 Fork head domain transcription factor AFX1 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 μg/mL.
Application
Western blot analysis of FOXO4 in HeLa cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | FOXO4 antibody can be used for detection of FOXO4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FOXO4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FOXO4 Antibody is supplied in PBS containing 0.02% sodium azide. |
