Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of AGPAT3 Antibody in A2058 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with AGPAT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | AGPAT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, 1-acylglycerol-3-phosphate O-acyltransferase 3, 1-AGP acyltransferase 3, 1-AGPAT 3, Lysophosphatidic acid acyltransferase gamma, LPAAT-gamma, AGPAT3, LPAAT3 |
| Product description | AGPAT3 is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. This protein may be an integral membrane protein.1) Leung,D.W. et.al., Front. Biosci. 6, D944-D953 (2001) |
| Immunogen | This AGPAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the Central region of human AGPAT3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AGPAT3 |
|---|---|
| Alternative Names | 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma 1-acylglycerol-3-phosphate O-acyltransferase 3 1-AGP acyltransferase 3 1-AGPAT 3 Lysophosphatidic acid acyltransferase gamma LPAAT-gamma AGPAT3 LPAAT3 |
| Molecular Weight(MW) | 43 kDa |
Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of AGPAT3 Antibody in A2058 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with AGPAT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
