Application
Western blot analysis of AGPAT4 Antibody in MDA-MB435 cell line lysates (35ug/lane)Application
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AGPAT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1-acyl-sn-glycerol-3-phosphate acyltransferase delta, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1-AGP acyltransferase 4, 1-AGPAT 4, Lysophosphatidic acid acyltransferase delta, LPAAT-delta, AGPAT4 |
| Product description | AGPAT4 converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.1) Leung,D.W. et.al., Front. Biosci. 6, D944-D953 (2001) |
| Immunogen | This AGPAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-202 amino acids from the Central region of human AGPAT4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AGPAT4 |
|---|---|
| Alternative Names | 1-acyl-sn-glycerol-3-phosphate acyltransferase delta 1-acylglycerol-3-phosphate O-acyltransferase 4 1-AGP acyltransferase 4 1-AGPAT 4 Lysophosphatidic acid acyltransferase delta LPAAT-delta AGPAT4 |
| Molecular Weight(MW) | 44 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis of AGPAT4 Antibody in MDA-MB435 cell line lysates (35ug/lane)
Application
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
