Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Product Name | AGPAT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGPAT5, 1-AGPAT5, LPAAT-e, LPAAT-epsilon, LPAATE, 1AGPAT5 |
| Product description | Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis.1) Agarwal, A.K., Arch. Biochem. Biophys. 449 (1-2), 64-76 (2006). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AGPAT5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AGPAT5 antibody can be used for detection of AGPAT5 by ELISA at 1:1562500. AGPAT5 antibody can be used for detection of AGPAT5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AGPAT5 |
|---|---|
| Alternative Names | AGPAT5 1-AGPAT5 LPAAT-e LPAAT-epsilon LPAATE 1AGPAT5 |
| Molecular Weight(MW) | 42 kDa |
Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | AGPAT5 antibody can be used for detection of AGPAT5 by ELISA at 1:1562500. AGPAT5 antibody can be used for detection of AGPAT5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AGPAT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
