hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development. Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.
Immunogen
AGR2 antibody was raised against amino acids 55 - 72 of AGR2 (Human).
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHC
AGR2 antibody can be used in ELISA starting at 1:3000 - 1:12000, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 5 μg/mL.:
Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity to their species of interest. Does not recognize AGR3 (Verma et al, 2012).
Immunohistochemistry staining of AGR2 in colon tissue using AGR2 Antibody.
Application Notes
AGR2 antibody can be used in ELISA starting at 1:3000 - 1:12000, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 5 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
Store AGR2 antibody at 4 ˚C for short term applications. For long term storage, aliquot and -20 ˚C.