Application
Western blot analysis of AGXT Antibody in HepG2 cell line lysates (35ug/lane)(8ug/ml)Application
Antibody staining AGXT in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:8000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.Application
AGXT Antibody (RB18848) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | AGXT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine--pyruvate aminotransferase, SPT, Alanine--glyoxylate aminotransferase, AGT, AGXT, AGT1, SPAT |
| Product description | AGXT is expressed only in the liver and protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification.1) Cellini,B., J. Biol. Chem. 284 (13), 8349-8358 (2009) |
| Immunogen | This AGXT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human AGXT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AGXT |
|---|---|
| Alternative Names | Serine--pyruvate aminotransferase SPT Alanine--glyoxylate aminotransferase AGT AGXT AGT1 SPAT |
| Molecular Weight(MW) | 43 kDa |
Application
Western blot analysis of AGXT Antibody in HepG2 cell line lysates (35ug/lane)(8ug/ml)
Application
Antibody staining AGXT in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:8000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
Application
AGXT Antibody (RB18848) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
