Application
'Western Blot: Profilin Antibody -Detection of Profilin in HeLa WCL (30 ug) using ; 2 min; ECL exposure. Lane 1: 0.1 ug/ml Lane 2: 0.5 ug/ml'| Product Name | Profilin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Profilin 1The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is believed to regulate actin polymerization in response to extracellular signalling. Deletion of this gene is associated with Miller-Dieker syndrome. |
| Immunogen | Porcine and human platelet profilin affinity purified on the antigen. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1-0.2 μg/ml. Detects a band of approximately 12-15 kDa (predicted molecular weight: 16.8 kDa). Use tricine gels (13% acrylamide) or 15% Laemmli gels. The given concentrations refer to the analysis of mammalian cells and tissues with intermediate levels of profilin expression. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatMarsupials |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P07737 PFN1 176610 5216 Profilin 1 Profilin1 |
|---|---|
| Target | Profilin 1 |
| Tissue Specificity | This product is specific for profilin 1 protein but also reacts with profilin 2. |
Application
'Western Blot: Profilin Antibody -Detection of Profilin in HeLa WCL (30 ug) using ; 2 min; ECL exposure. Lane 1: 0.1 ug/ml Lane 2: 0.5 ug/ml'| Positive Control | Human platelet protein (25ug/lane) - GTX82934 |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1-0.2 μg/ml. Detects a band of approximately 12-15 kDa (predicted molecular weight: 16.8 kDa). Use tricine gels (13% acrylamide) or 15% Laemmli gels. The given concentrations refer to the analysis of mammalian cells and tissues with intermediate levels of profilin expression. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS containing 1mg/ml BSA and 0.01% sodium azide |
