Application
Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using Profilin 1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 15% SDS PAGE diluted at 1:1000Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 1 antibody [N1C3] diluted at 1:500.Application
Profilin 1 antibody detects Profilin 1 protein by Western blot analysis. A. 50 µg mouse spleen lysate/extract 15 % SDS-PAGE Profilin 1 antibody dilution: 1:500| Product Name | Profilin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Profilin 1 (profilin 1)The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Profilin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (94%), Bovine (93%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PFN1 antibody profilin-1 antibody profilin I antibody profilin 1 antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using Profilin 1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 15% SDS PAGE diluted at 1:1000
Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 1 antibody [N1C3] diluted at 1:500.
Application
Profilin 1 antibody detects Profilin 1 protein by Western blot analysis. A. 50 µg mouse spleen lysate/extract 15 % SDS-PAGE Profilin 1 antibody dilution: 1:500| Positive Control | 293T , A431 , HeLa , HepG2 , mouse spleen , Rat spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
