| Product Name | Progesterone antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ProgesteroneProgesterone Levels1. Men 30-60 pg/0.1ml2. WomenPre-Ovulatory Phase: 20-160 pg/0.1mlOvulatory Phase: 1,000-1,700 pg/0.1mlPost-Ovulatory Phase: 1,000-1,700 pg/0.1mlPregnant:16-18 weeks: 300-800 pg/0.1ml28-30 weeks: 6,500-14,700 pg/0.1ml38-40 weeks: 12,000-19,000 pg/0.1ml |
| Immunogen | Full length native Progesterone 11 (purified) (Human) conjugated to BSA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | RIA |
| RIA: The antiserum is characterized utilizing the following dextran coated charcoal radioimmunoassay (RIA) protocol, where 0.5 ml of reconstituted and diluted antiserum has been found to bind at least 40% of 10-15 picograms of tritiated (^3H) progesterone with a specific activity of approximately 100 Ci/mmole. RIA Reagents(A) Standards: Prepare a stock standard solution of 1 μg/ml progesterone in absolute ethanol. Dilute a portion of the stock solution with buffer (B) to a concentration of 500 pg/0.1ml. This is further diluted in buffer (B) to obtain standard solutions at the following concentrations: 250, 125, 63, 31 and 15 pg/0.1 ml.(B) Dilution buffer: 0.05 M Tris-HCl, pH 8.0, containing 0.1 M NaCl, 0.1% BSA and 0.1% sodium azide.(C) Dextran coated charcoal suspension: 0.5% activated charcoal untreated powder 100-400 mesh, 0.5% dextran approximate average molecular weight 70,000 in buffer (B). It is important that the dextran be in solution before the addition of charcoal. The dextran coated charcoal suspension should be stirred and kept at 0°C in ice-water for at least 30 minutes before and during use.1. In polypropylene test tubes add 0.1 ml sample or standard (A) and 0.5 ml diluted antiserum.2. Vortex the tubes.3. Incubate for 30 minutes at room temperature.4. Add 0.1 ml tritiated radioactive tracer diluted in dilution buffer (B).5. Vortex the tubes.6. Incubate for 1 hour at 37°C.7. Cool the tubes for 15 minutes at 4°C.8. Rapidly add 0.2 ml cold dextran coated charcoal suspension (C) to each tube.9. Vortex the tubes.10. Incubate for 10 minutes at 0°C in ice-water.11. Centrifuge at 2000 x g for 15 minutes at 4°C. Sensitivity is defined as the 90% intercept of a B/B0 standard curve. In the above system the sensitivity has been found to be 5pg progesterone/tube. The affinity constant (K ) is determined by a Scatchard a plot using the described RIA system. K = 2.9 x 10 L/mole. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Progesterone |
|---|---|
| Tissue Specificity | Specificity of the antiserum is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in the described RIA system is as follows:5 alpha-Pregnane-3,20-dione 1.8%3 beta-Hydroxy-5-pregnane-20-one 22.2%Corticosterone |
| Application Notes | RIA: The antiserum is characterized utilizing the following dextran coated charcoal radioimmunoassay (RIA) protocol, where 0.5 ml of reconstituted and diluted antiserum has been found to bind at least 40% of 10-15 picograms of tritiated (^3H) progesterone with a specific activity of approximately 100 Ci/mmole. RIA Reagents(A) Standards: Prepare a stock standard solution of 1 μg/ml progesterone in absolute ethanol. Dilute a portion of the stock solution with buffer (B) to a concentration of 500 pg/0.1ml. This is further diluted in buffer (B) to obtain standard solutions at the following concentrations: 250, 125, 63, 31 and 15 pg/0.1 ml.(B) Dilution buffer: 0.05 M Tris-HCl, pH 8.0, containing 0.1 M NaCl, 0.1% BSA and 0.1% sodium azide.(C) Dextran coated charcoal suspension: 0.5% activated charcoal untreated powder 100-400 mesh, 0.5% dextran approximate average molecular weight 70,000 in buffer (B). It is important that the dextran be in solution before the addition of charcoal. The dextran coated charcoal suspension should be stirred and kept at 0°C in ice-water for at least 30 minutes before and during use.1. In polypropylene test tubes add 0.1 ml sample or standard (A) and 0.5 ml diluted antiserum.2. Vortex the tubes.3. Incubate for 30 minutes at room temperature.4. Add 0.1 ml tritiated radioactive tracer diluted in dilution buffer (B).5. Vortex the tubes.6. Incubate for 1 hour at 37°C.7. Cool the tubes for 15 minutes at 4°C.8. Rapidly add 0.2 ml cold dextran coated charcoal suspension (C) to each tube.9. Vortex the tubes.10. Incubate for 10 minutes at 0°C in ice-water.11. Centrifuge at 2000 x g for 15 minutes at 4°C. Sensitivity is defined as the 90% intercept of a B/B0 standard curve. In the above system the sensitivity has been found to be 5pg progesterone/tube. The affinity constant (K ) is determined by a Scatchard a plot using the described RIA system. K = 2.9 x 10 L/mole. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC - lyophilized. After reconstitution store aliquots at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None, Constituents: Whole Serum |
