Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 2 antibody diluted at 1:1000.Application
Sample(30 ug whole cell lysate) A: A431 B: H1299 12% SDS PAGE diluted at 1:500Application
Profilin 2 antibody detects PFN2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Profilin 2 antibody dilution: 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000| Product Name | Profilin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Profilin 2 (profilin 2)The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Profilin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, BovineChicken (92%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | D3S1319E antibody PFL antibody PFN2 antibody profilin II antibody profilin-2 antibody profilin 2 antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Tissue Specificity | This antibody may cross-react with human Profilin 1. |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 2 antibody diluted at 1:1000.
Application
Sample(30 ug whole cell lysate) A: A431 B: H1299 12% SDS PAGE diluted at 1:500
Application
Profilin 2 antibody detects PFN2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Profilin 2 antibody dilution: 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000| Positive Control | A431 , H1299 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
