AHCYL1 belongs to the adenosylhomocysteinase family.The protein, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/HCO3- cotransporter 1 (pNBC1).The regulation through AHCYL1 enables NBC1 variants to have different physiological roles.1) Cooper, B.J., (2006) J. Biol. Chem. 281 (32), 22471-22484.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AHCYL1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
AHCYL1 antibody can be used for detection of AHCYL1 by ELISA at 1:32500. AHCYL1 antibody can be used for detection of AHCYL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
AHCYL1 antibody can be used for detection of AHCYL1 by ELISA at 1:32500. AHCYL1 antibody can be used for detection of AHCYL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AHCYL1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.