Application
Immunofluorescent analysis of Ser190-phosphorylated Progesterone Receptor in untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Progesterone Receptor (phospho Ser190) antibody [1154] at a dilution of 1:50 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.| Product Name | Progesterone Receptor (phospho Ser190) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1154] to Ser190-phosphorylated Progesterone ReceptorThis gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011] |
| Immunogen | Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor. |
| Modification | p-Ser190 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1154 |
| Host Species | Mouse |
| Tested Applications | ELISAGSAICC/IFIHC-PIPWB |
| IHC-P: 2-4 ug/ml: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | PGR progesterone receptor PR NR3C3 |
|---|---|
| Target | Phospho-Progesterone Receptor pSer190 |
Application
Immunofluorescent analysis of Ser190-phosphorylated Progesterone Receptor in untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Progesterone Receptor (phospho Ser190) antibody [1154] at a dilution of 1:50 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.| Application Notes | IHC-P: 2-4 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Contains 0.05 % sodium azide and 1 mg/ml BSA |
