Application
Application| Product Name | Progesterone Receptor (phospho Ser190) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1154] to Progesterone Receptor (phospho Ser190)The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120kDa protein (Form B) or a 94kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser190 conjugated to KLH |
| Modification | p-Ser190 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1154 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | P06401 PR 5241 PGR 607311 NR3C3 Progesterone Receptor (phospho S190) Progesterone Receptor (phospho Ser190) Progesterone Receptor (pS190) Progesterone Receptor (pSer190) Progesterone Receptor phospho S190 Progesterone Receptor phospho Ser190 Ph |
|---|---|
| Target | Progesterone Receptor (phospho Ser190) |
| Molecular Weight(MW) | 90/120 (note) |
| Cellular Localization | Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases. Mainly nuclear. |
Application
Application| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
