Application
Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294 using Progesterone Receptor (phospho Ser294) antibody [608] . The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).| Product Name | Progesterone Receptor (phospho Ser294) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [608] to Progesterone Receptor (phospho Ser294)The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120kDa protein (Form B) or a 94kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH |
| Modification | p-Ser294 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 608 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | PGR 607311 P06401 PR NR3C3 5241 Progesterone Receptor (phospho S294) Progesterone Receptor (phospho Ser294) Progesterone Receptor (pS294) Progesterone Receptor (pSer294) Progesterone Receptor phospho S294 Progesterone Receptor phospho Ser294 Ph |
|---|---|
| Target | Progesterone Receptor (phospho Ser294) |
| Molecular Weight(MW) | 90/120 (note) |
| Cellular Localization | Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases. Mainly nuclear. |
Application
Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294 using Progesterone Receptor (phospho Ser294) antibody [608] . The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
