Application
Immunohistochemistry of AIF in human retina tissue with AIF antibody at 10 μg/mL.Application
Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 μg/mL.| Product Name | AIF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1 |
| Product description | AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.1) Zamzami N and Kroemer G. Condensed matter in cell death. Nature 1999; 401:127-8. |
| Immunogen | AIF antibody was raised against a 15 amino acid peptide near the center of human AIF. The immunogen is located within amino acids 500 - 550 of AIF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| AIF antibody can be used for detection of AIF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CNKSR1 |
|---|---|
| Alternative Names | AIF Antibody: CNK KSR CNK1 Connector enhancer of kinase suppressor of ras 1 CNK homolog protein 1 Connector enhancer of KSR 1 |
| Molecular Weight(MW) | Predicted: 27, 36, 67 kDa Observed: 71 kDa |
| Sequence Similarities | 3) Cat. No. 1401 - Mouse Heart Tissue Lysate |
| Tissue Specificity | At least five isoforms of AIF are known to exist; this antibody will detect all isoforms except isoform 5. |
Application
Immunohistochemistry of AIF in human retina tissue with AIF antibody at 10 μg/mL.
Application
Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | AIF antibody can be used for detection of AIF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AIF Antibody is supplied in PBS containing 0.02% sodium azide. |
