Application
Immunohistochemistry of AIM in human lymph node tissue with AIM antibody at 2 μg/mL.Application
Immunofluorescence of AIM in Human Lymph Node cells with AIM antibody at 20 μg/mL.Application
Western blot analysis of AIM in human lymph node tissue lysate with AIM antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Product Name | AIM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIM Antibody: AIM, API6, PRO229, Spalpha, SP-ALPHA, UNQ203/PRO229, CD5 antigen-like, CT-2 |
| Product description | AIM Antibody: Apoptosis inhibitor of macrophages (AIM) is a member of the scavenger receptor cysteine-rich domain superfamily (SRCR-SF) initially identified as an inducible cell surface ligand of CD5. It was shown that AIM functions in the thymus as the inducer of resistance to apoptosis within CD4+/CD8+ thymocytes and as the supporter of the viability of these cells before thymic selection. AIM was also shown to support macrophage survival and enhance their phagocytic function. More recent experiments using recombinant AIM significantly inhibited apoptosis of NKT and T cells obtained from C. parvum-stimulated livers in vitro, suggesting that AIM functions to induce resistance to apoptosis in these cells and supports host defense against inflammation during infection.1) Miyazaki T, Hirokami Y, Matsuhashi N, et al. Increased susceptibility of thymocytes to apoptosis in mice lacking AIM, a novel murine macrophage-derived soluble factor belonging to the scavenger receptor cysteine-rich domain superfamily. J. Exp. Med. 1999; 189:413-22. |
| Immunogen | AIM antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human AIM. The immunogen is located within amino acids 60 - 110 of AIM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AIM antibody can be used for the detection of AIM by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD5L |
|---|---|
| Alternative Names | AIM Antibody: AIM API6 PRO229 Spalpha SP-ALPHA UNQ203/PRO229 CD5 antigen-like CT-2 |
| Sequence Similarities | 2) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Application
Immunohistochemistry of AIM in human lymph node tissue with AIM antibody at 2 μg/mL.
Application
Immunofluorescence of AIM in Human Lymph Node cells with AIM antibody at 20 μg/mL.
Application
Western blot analysis of AIM in human lymph node tissue lysate with AIM antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1369 - Human Lymph node Tissue Lysate |
|---|---|
| Application Notes | AIM antibody can be used for the detection of AIM by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AIM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AIM Antibody is supplied in PBS containing 0.02% sodium azide. |
