Application
Prohibitin 2 antibody detects PHB2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Prohibitin 2 antibody dilution: 1:500.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA antibody at 1:200 dilution.Application
Prohibitin 2 antibody detects Prohibitin 2 protein at cytoplasm and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder . Prohibitin 2 antibody diluted at 1:500.| Product Name | Prohibitin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Prohibitin 2 (prohibitin 2)Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Prohibitin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineXenopus laevis (86%), Pig (98%), Chicken (94%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAP antibody BCAP37 antibody Bap37 antibody MGC117268 antibody PNAS-141 antibody REA antibody p22 antibody PHB2 antibody B-cell receptor-associated protein BAP37 antibody prohibitin-2 antibody B-cell associated protein antibody D-prohibitin anti |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (3) |
| Cellular Localization | Mitochondrion inner membrane (By similarity) , Cytoplasm (By similarity) , Nucleus (By similarity) |
Application
Prohibitin 2 antibody detects PHB2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Prohibitin 2 antibody dilution: 1:500.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA antibody at 1:200 dilution.
Application
Prohibitin 2 antibody detects Prohibitin 2 protein at cytoplasm and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder . Prohibitin 2 antibody diluted at 1:500.| Positive Control | H1299 , HeLa , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
