Application
Western blot analysis of Aipl1 in rat brain tissue lysate with Aipl1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of Aipl1 in human brain tissue with Aipl1 antibody at 2.5 μg/mL.Application
Immunofluorescence of aipl1 in human brain tissue with aipl1 antibody at 20 μg/mL.| Product Name | Aipl1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aipl1 Antibody: LCA4, AIPL2, Aryl-hydrocarbon-interacting protein-like 1 |
| Product description | Aipl1 Antibody: Aipl1 was initially identified as a protein implicated in Leber congenital amaurosis (LCA), an autosomal recessive disorder thought to be caused by the abnormal development of photoreceptors. Aipl1 is a tetratricopeptide repeat protein that is highly homologous to ARA9, a protein involved in the HSP90-mediated nuclear translocation and transactivation of the aryl hydrocarbon receptor. Aipl1 has also been found to function as part of a chaperone heterocomplex, interacting with Hsp90 and Hsp70. Aipl1 also associates with the cell cycle regulator NUB1. It is thought that Aipl1 cooperates with Hsp70 but not Hsp90 to suppress the formation of NUB1 inclusions, and these interactions are necessary in the normal photoreceptor maturation, as mutations that lead to LCA also compromise the interactions with the Hsp chaperones. At least three isoforms of Aipl1 are known to exist.1) Sohocki MM, Brown SJ, Sullivan LS, et al. Mutations in a new photoreceptor-pineal gene on 17p cause Leber congenital amaurosis. Nat. Genet.2000; 24:79-83. |
| Immunogen | Aipl1 antibody was raised against a 18 amino acid synthetic peptide near the center of the human Aipl1. The immunogen is located within amino acids 140 - 190 of Aipl1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Aipl1 antibody can be used for detection of Aipl1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AIPL1 |
|---|---|
| Alternative Names | Aipl1 Antibody: LCA4 AIPL2 Aryl-hydrocarbon-interacting protein-like 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of Aipl1 in rat brain tissue lysate with Aipl1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of Aipl1 in human brain tissue with Aipl1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of aipl1 in human brain tissue with aipl1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | Aipl1 antibody can be used for detection of Aipl1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aipl1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Aipl1 Antibody is supplied in PBS containing 0.02% sodium azide. |
