Application
Immunohistochemistry of AKAP4 in human testis tissue with AKAP4 antibody at 5 μg/ml.Application
Western blot analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of AKAP4 in human testis tissue with AKAP4 antibody at 20 μg/ml.| Product Name | AKAP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A-kinase anchor protein 4, AKAP-4, AKAP82, hAKAP82, CT99, FSC1, HI, PRKA4 |
| Product description | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1). AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2). It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2). AKAP4 has also been postulated to be a potential biomarker for breast cancer (3).1) Carnegie GK, Means CK, and Scott JD. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB 2009; 61:394-406. |
| Immunogen | AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4. The immunogen is located within the first 50 amino acids of AKAP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKAP4 |
|---|---|
| Alternative Names | A-kinase anchor protein 4 AKAP-4 AKAP82 hAKAP82 CT99 FSC1 HI PRKA4 |
| Molecular Weight(MW) | Predicted: 94 kDa Observed: 100 kDa |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms. |
Application
Immunohistochemistry of AKAP4 in human testis tissue with AKAP4 antibody at 5 μg/ml.
Application
Western blot analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of AKAP4 in human testis tissue with AKAP4 antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AKAP4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | AKAP4 antibody is supplied in PBS containing 0.02% sodium azide. |
