Application
Western blot analysis of Akirin2 in mouse brain tissue lysate with Akirin2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | Akirin2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Akirin2 Antibody: FBI1, C6orf166, dJ486L4.2, Akirin-2 |
| Product description | Akirin2 Antibody: The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-κB-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-κB pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-α and IL-1β signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous null D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.1) Goto A, Matsushita K, Gesellchen V, et al. Akirins are highly conserved nuclear proteins required for NF-kappaB-dependent gene expression in drosophila and mice. Nat. Immunol.2008; 9:97-104. |
| Immunogen | Akirin2 antibody was raised against a 15 amino acid synthetic peptide near the center of the human Akirin2. The immunogen is located within amino acids 50 - 100 of Akirin2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Akirin2 antibody can be used for detection of Akirin2 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKIRIN2 |
|---|---|
| Alternative Names | Akirin2 Antibody: FBI1 C6orf166 dJ486L4.2 Akirin-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (100%) |
Application
Western blot analysis of Akirin2 in mouse brain tissue lysate with Akirin2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | Akirin2 antibody can be used for detection of Akirin2 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Akirin2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Akirin2 Antibody is supplied in PBS containing 0.02% sodium azide. |
