Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).| Product Name | AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) Antibody |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, PKD, PKD1, PRKCM, RAC-PK-alpha, Protein kinase B |
| Product description | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.1) Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738. Malabarba M G, et al. (1996) Biochem. J. 319:865-872. Hou J, et al. (1994) Science. 265:1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343. |
| Immunogen | AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y (p) -L-A) derived from Human AKT1/AKT2/AKT3. |
| Modification | p-Tyr315 / Tyr316 / Tyr312 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 AKT2 AKT3 |
|---|---|
| Alternative Names | AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA PKD PKD1 PRKCM RAC-PK-alpha Protein kinase B |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312. |
| Entrez Gene | 207 / 208 / 10000 |
|---|---|
| Protein Accession | NP_001014431.1 / NP_001617.1 / NP_005456.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).
Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
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| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
