Application
Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.Application
Immunohistochemistry of AKT1S1 in rat brain tissue with AKT1S1 antibody at 2.5 μg/ml.| Product Name | AKT1S1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT1S1 Antibody: Lobe, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate |
| Product description | AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).1) Saito A, Narasimhan P, Hayashi T, et al. Neuroprotective role of a proline-rich Akt substrate in apoptotic neuronal cell death after stroke: relationships with nerve growth factor. J. Neurosci. 2004; 24:1584-93. |
| Immunogen | AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1. The immunogen is located within amino acids 190 - 240 of AKT1S1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1S1 |
|---|---|
| Alternative Names | AKT1S1 Antibody: Lobe PRAS40 Proline-rich AKT1 substrate 1 40 kDa proline-rich AKT substrate |
| Molecular Weight(MW) | Predicted: 30 kDa Observed: 28 kDa |
| Tissue Specificity | AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist. |
Application
Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.
Application
Immunohistochemistry of AKT1S1 in rat brain tissue with AKT1S1 antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AKT1S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide. |
