Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | AKT1S1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT1S1, Lobe, MGC2865, PRAS40 |
| Product description | AKT1S1 may play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. It is the substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. AKT1S1 may also play a role in nerve growth factor-mediated neuroprotection.AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).1) Fonseca, B.D., (2008) Biochem. J. 411 (1), 141-149. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1S1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AKT1S1 antibody can be used for detection of AKT1S1 by ELISA at 1:62500. AKT1S1 antibody can be used for detection of AKT1S1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1S1 |
|---|---|
| Alternative Names | AKT1S1 Lobe MGC2865 PRAS40 |
| Molecular Weight(MW) | 27 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | AKT1S1 antibody can be used for detection of AKT1S1 by ELISA at 1:62500. AKT1S1 antibody can be used for detection of AKT1S1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AKT1S1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
