Application
Western blot of proteasome 19S subunit S4 on HeLa cell lysate using| Product Name | Proteasome 19S Subunit S4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 19S Subunit S4Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome. |
| Immunogen | Yeast proteasome 19S subunit S4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB: 1:1,000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMousePrimate |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P62191 MGC24583 PSMC1 P26S4 602706 p56 S4 Proteasome 19S S4 5700 MGC8541 Proteasome19S S4 |
|---|---|
| Target | Proteasome 19S Subunit S4 |
Application
Western blot of proteasome 19S subunit S4 on HeLa cell lysate using| Application Notes | WB: 1:1,000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
