Application
Proteasome 20S alpha 1 antibody [N1C3] detects Proteasome 20S alpha 1 protein at cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. Proteasome 20S alpha 1 antibody [N1C3] diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using 20S Proteasome alpha1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: U87-MG B: MCF-7 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Proteasome 20S alpha 1 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 20S alpha 1 (proteasome (prosome, macropain) subunit, alpha type, 1)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (95%), Xenopus laevis (95%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HC2 antibody MGC14542 antibody MGC14575 antibody MGC14751 antibody MGC1667 antibody MGC21459 antibody MGC22853 antibody MGC23915 antibody NU antibody PROS30 antibody PSMA1 antibody proteasome nu chain antibody protein P30-33K antibody macropai |
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| Molecular Weight(MW) | 30 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Proteasome 20S alpha 1 antibody [N1C3] detects Proteasome 20S alpha 1 protein at cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. Proteasome 20S alpha 1 antibody [N1C3] diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using 20S Proteasome alpha1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: U87-MG B: MCF-7 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , U87-MG , MCF-7 , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
