Application
Proteasome 20S alpha 3 antibody detects PSMA3 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 3 antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft , using 20S Proteasome alpha3 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000| Product Name | Proteasome 20S alpha 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 20S alpha 3 (proteasome (prosome, macropain) subunit, alpha type, 3)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (100%), Zebrafish (90%), Xenopus laevis (92%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HC8 antibody MGC12306 antibody MGC32631 antibody PSC3 antibody PSMA3 antibody proteasome subunit alpha type-3 antibody proteasome subunit C8 antibody multicatalytic endopeptidase complex subunit C8 antibody proteasome component C8 antibody macropa |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Proteasome 20S alpha 3 antibody detects PSMA3 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 3 antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft , using 20S Proteasome alpha3 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000| Positive Control | Molt-4 , NIH-3T3 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
