Application
Proteasome 20S beta 7 antibody [N2C3] detects Proteasome 20S beta 7 protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Proteasome 20S beta 7 antibody [N2C3] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Proteasome 20S beta 7 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using Proteasome 20S beta 7 antibody at 1:100 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Proteasome 20S beta 7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 20S beta 7 (proteasome (prosome, macropain) subunit, beta type, 7)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome 20S beta 7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Chicken, Rhesus Monkey, BovineRat (95%), Dog (95%), Pig (93%), Chicken (84%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Z antibody PSMB7 antibody proteasome subunit Z antibody macropain chain Z antibody proteasome subunit beta 7 antibody proteasome subunit beta type-7 antibody proteasome subunit alpha antibody multicatalytic endopeptidase complex chain Z antibody pr |
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| Molecular Weight(MW) | 30 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Proteasome 20S beta 7 antibody [N2C3] detects Proteasome 20S beta 7 protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Proteasome 20S beta 7 antibody [N2C3] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Proteasome 20S beta 7 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using Proteasome 20S beta 7 antibody at 1:100 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HeLa , HepG2 , HCT116 , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
