Application
Immunofluorescence analysis of methanol-fixed A431, using Proteasome 26S S3 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | Proteasome 26S S3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 26S S3 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 3)The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (99%), Rat (99%), Zebrafish (96%), Xenopus laevis (95%), Chicken (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P58 antibody RPN3 antibody S3 antibody TSTA2 antibody PSMD3 antibody 26S proteasome regulatory subunit S3 antibody tissue specific transplantation antigen 2 antibody 26S proteasome regulatory subunit RPN3 antibody proteasome subunit p58 antibody 2 |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed A431, using Proteasome 26S S3 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
