Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Proteasome 26S S3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE Proteasome 19S S3 antibody diluted at 1:1000| Product Name | Proteasome 26S S3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 26S S3 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 3)The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, Chimpanzee, BovineRat (93%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (96%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P58 antibody RPN3 antibody S3 antibody TSTA2 antibody PSMD3 antibody 26S proteasome regulatory subunit S3 antibody tissue specific transplantation antigen 2 antibody 26S proteasome regulatory subunit RPN3 antibody proteasome subunit p58 antibody 2 |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Proteasome 26S S3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE Proteasome 19S S3 antibody diluted at 1:1000| Positive Control | A549 , Hela , Jurkat , NT2D1 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
