Application
Western blot analysis of lysates from human heart tissue lysate, U-251 MG cell line (from left to right), using AKTIP Antibody at 1:1000 at each lane.| Product Name | AKTIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT-interacting protein, Ft1, Fused toes protein homolog, AKTIP, FTS |
| Product description | The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.1) Notaridou, M., et al. Int. J. Cancer (2010) In press : |
| Immunogen | This AKTIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-288 amino acids from the C-terminal region of human AKTIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKTIP |
|---|---|
| Alternative Names | AKT-interacting protein Ft1 Fused toes protein homolog AKTIP FTS |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis of lysates from human heart tissue lysate, U-251 MG cell line (from left to right), using AKTIP Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
