Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane)| Product Name | AlaRS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alanine--tRNA ligase, cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, Renal carcinoma antigen NY-REN-42, AARS {ECO:000 |
| Product description | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.1) Shiba,K.,Biochemistry 34 (33), 10340-10349 (1995) |
| Immunogen | This AlaRS (AARS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human AlaRS (AARS). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AARS |
|---|---|
| Alternative Names | Alanine--tRNA ligase cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133} 6117 {ECO:0000255|HAMAP-Rule:MF_03133} Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133} AlaRS {ECO:0000255|HAMAP-Rule:MF_03133} Renal carcinoma antigen NY-REN-42 AARS {ECO:000 |
| Molecular Weight(MW) | 107 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: C. Elegans |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
