WB
Western blot analysis of AlaRS/AARS1 using anti-AlaRS/AARS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AlaRS/AARS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of AlaRS/AARS1 using anti-AlaRS/AARS1 antibody. AlaRS/AARS1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-AlaRS/AARS1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of K562 cells using anti-AlaRS/AARS1 antibody . Overlay histogram showing K562 cells stained with Rabbit polyclonal antibody to AlaRS/AARS1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AlaRS/AARS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to AlaRS/AARS1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human AlaRS/AARS1 recombinant protein (Position: R729-N968). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AARS1 |
|---|---|
| Gene Synonyms | AARS TTD8 CMT2N DEE29 HDLS2 EIEE29 |
| Gene Full Name | alanyl-tRNA synthetase 1 |
| Gene Summary | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
| Alternative Names | AARS Alanine tRNA ligase alanyl tRNA synthetase AlaRS |
| Molecular Weight(MW) | 107 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
| SwissProt ID | P49588 |
|---|
WB
Western blot analysis of AlaRS/AARS1 using anti-AlaRS/AARS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AlaRS/AARS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of AlaRS/AARS1 using anti-AlaRS/AARS1 antibody. AlaRS/AARS1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-AlaRS/AARS1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of K562 cells using anti-AlaRS/AARS1 antibody . Overlay histogram showing K562 cells stained with Rabbit polyclonal antibody to AlaRS/AARS1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AlaRS/AARS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
