Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH18A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of ALDH18A1 Antibody in K562 cell line lysates (35ug/lane)| Product Name | ALDH18A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Delta-1-pyrroline-5-carboxylate synthase, P5CS, Aldehyde dehydrogenase family 18 member A1, Glutamate 5-kinase, GK, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase, GPR, Glutamate-5-semialdehyde dehydrogenase, Glutamyl-gamma-semialdehyde dehydrog |
| Product description | ALDH18A1 is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine.1) Hu,C.A., et.al., Amino Acids 35 (4), 665-672 (2008) |
| Immunogen | This ALDH18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the Central region of human ALDH18A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALDH18A1 |
|---|---|
| Alternative Names | Delta-1-pyrroline-5-carboxylate synthase P5CS Aldehyde dehydrogenase family 18 member A1 Glutamate 5-kinase GK Gamma-glutamyl kinase Gamma-glutamyl phosphate reductase GPR Glutamate-5-semialdehyde dehydrogenase Glutamyl-gamma-semialdehyde dehydrog |
| Molecular Weight(MW) | 87 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH18A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of ALDH18A1 Antibody in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
