Application
Fluorescent confocal image of A549 cell stained with ALDH2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PApplication
Western blot analysis in A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35ug/lane).| Product Name | ALDH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM |
| Product description | ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.1) Guo,Y.M., World J. Gastroenterol. 14 (9), 1444-1449 (2008) Chen,L., PLoS Med.(3), E52 (2008) Teeguarden,J.G., Inhal Toxicol 20 (4), 375-390 (2008) Yoshida,A., Pharmacogenetics(4), 139-147 (1992) |
| Immunogen | This ALDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human ALDH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALDH2 |
|---|---|
| Alternative Names | Aldehyde dehydrogenase mitochondrial ALDH class 2 ALDH-E2 ALDHI ALDH2 ALDM |
| Molecular Weight(MW) | 56 kDa |
Application
Fluorescent confocal image of A549 cell stained with ALDH2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated P
Application
Western blot analysis in A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
