Application
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in A549,HepG2 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ALDH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM |
| Product description | ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.1) Guo,Y.M., World J. Gastroenterol. 14 (9), 1444-1449 (2008) |
| Immunogen | This ALDH2 antibody is generated from rabbits immunized with human ALDH2 recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALDH2 |
|---|---|
| Alternative Names | Aldehyde dehydrogenase mitochondrial ALDH class 2 ALDH-E2 ALDHI ALDH2 ALDM |
| Molecular Weight(MW) | 56 kDa |
Application
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in A549,HepG2 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
