Application
Western blot analysis of ALG14 Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALG14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ALG14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UDP-N-acetylglucosamine transferase subunit ALG14 homolog, ALG14 |
| Product description | ALG14 is involved in protein N-glycosylation. It is essential for the second step of the dolichol-linked oligosaccharide pathway. It anchors the catalytic subunit ALG13 to the ER.1) Gao X.-D., et.al., J. Biol. Chem. 280:36254-36262(2005). |
| Immunogen | This ALG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the Central region of human ALG14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALG14 |
|---|---|
| Alternative Names | UDP-N-acetylglucosamine transferase subunit ALG14 homolog ALG14 |
| Molecular Weight(MW) | 24 kDa |
Application
Western blot analysis of ALG14 Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALG14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
