Application
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).Application
ALKBH6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ALKBH6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6, 11411-, Alkylated DNA repair protein alkB homolog 6, ALKBH6, ABH6 |
| Product description | Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).1) Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002) |
| Immunogen | This ALKBH6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the Central region of human ALKBH6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALKBH6 |
|---|---|
| Alternative Names | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 11411- Alkylated DNA repair protein alkB homolog 6 ALKBH6 ABH6 |
| Molecular Weight(MW) | 26 kDa |
Application
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Application
ALKBH6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
