Application
Western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of ALOX15 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ALOX15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arachidonate 15-lipoxygenase, 15-LOX, 15-LOX-1, 12/15-lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, 12-LOX, Arachidonate omega-6 lipoxygenase, ALOX15, LOG15 |
| Product description | ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.1) Tang,Y., Int. J. Cancer 124 (7), 1545-1551 (2009) |
| Immunogen | This ALOX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ALOX15 |
|---|---|
| Alternative Names | Arachidonate 15-lipoxygenase 15-LOX 15-LOX-1 12/15-lipoxygenase Arachidonate 12-lipoxygenase leukocyte-type 12-LOX Arachidonate omega-6 lipoxygenase ALOX15 LOG15 |
| Molecular Weight(MW) | 75 kDa |
Application
Western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of ALOX15 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
