Application
Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 2.5 μg/ml.Application
Immunofluorescence of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 20 μg/mL.Application
Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 5 μg/mL.Application
Western blot analysis of alpha-Tubulin in 293, Daudi, Hela, HepG2, Jurkat, K562, NH3T3, Raji, Ramos, U937, Human brain, Mouse brain, Rat Brain, Rabbit Brain, Rabbit Spleen, Zebrafish, Mouse Liver and Chicken liver lysate at 1 μg/mL.| Product Name | alpha-Tubulin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tubulin alpha-1A, TUBA1A, TUBA3, LIS3 |
| Product description | The alpha-Tubulin antibody belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, tubulin alpha-1A are the major components of microtubules. Like GAPDH and B-Actin, this TUBA1A antibody makes an excellent loading control in immunoblots.1) McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33. |
| Immunogen | alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TUBA1A |
|---|---|
| Alternative Names | Tubulin alpha-1A TUBA1A TUBA3 LIS3 |
| Molecular Weight(MW) | Predicted: 50 kDa Observed: 54 kDa |
| Tissue Specificity | alpha-Tubulin antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 2.5 μg/ml.
Application
Immunofluorescence of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 20 μg/mL.
Application
Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 5 μg/mL.
Application
Western blot analysis of alpha-Tubulin in 293, Daudi, Hela, HepG2, Jurkat, K562, NH3T3, Raji, Ramos, U937, Human brain, Mouse brain, Rat Brain, Rabbit Brain, Rabbit Spleen, Zebrafish, Mouse Liver and Chicken liver lysate at 1 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | alpha-Tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide. |
